Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression
نویسندگان
چکیده
منابع مشابه
Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression.
Concomitant activity improvement of an evolved enzyme toward two very different ester substrates was achieved when a unique combination of functional periplasmic enzyme expression in Escherichia coli, random mutagenesis, DNA shuffling and cell-based kinetic screenings was applied. Specifically, we focused on the conversion of subtilisin E into an enzyme with broader esterase activity as opposed...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2001
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/14.11.929